IMAGE GALLERY

Lecture in Kyoto, Japan

Guests of the International Nishinomiya-Yukawa Symposium, Kyoto, Japan.

Calbindin-positive cells in auditory area NCM are a subset of GABAergic neurons, and do not exhibit song-evoked regulation of the immediate early gene zenk. From Pinaud et al., 2006.

Song-evoked multi-unitary responses obtained from two sites within auditory nucleus NCM of an awake zebra finch. The bottom trace corresponds to the amplitude envelope of the sound stimulus (a conspecific song). From Pinaud et al., 2008a.

Representative 2D-DIGE gel (left) illustrating fractionated proteins from the songbird auditory forebrain in a comparison between controls (unstimulated) and birds that experienced 1-hour of auditory stimulation. The control sample is labeled with the fluorophore Cy3 (green) while the experimental sample is labeled with Cy5 (red). Internal control samples are labeled with Cy2 (blue; not shown). The Comassie blue-stained gel on the right illustrates experience-regulated proteins (arrows) in the experimental group, as revealed by 2D-DIGE-based quantitative proteomics and statistical analyses. The identity of all experience-regulated proteins was determined by mass-spectrometry fingerprinting. From Pinaud et al., 2008b.

Frequency tuning curves obtained from NCM units in the awake zebra finch. Blue traces correspond to pre-drug frequency tuning functions whereas red traces illustrate the tuning functions for those some neurons after local application of bicuculline, a GABA-A receptor antagonist.

Cells expressing zGAD65, the zebra finch homologue of the 65 KDa glutamic acid decarboxylase gene, indicating a prevalence of inhbitory neurons in auditory nucleus NCM. From Pinaud et al., 2004.

Calbindin-positive cell soma and corresponding immunolabeled neurites in the zebra finch caudal pallium. From Pinaud et al., 2007a.

Whole-cell patch clamp (current clamp) recordings obtained from three individual neurons in auditory area NCM. Examples of action potentials from a tonically-responding cell (A), tonically-adapting (B) and phasic (C) neurons. From Pinaud et al., 2008a.

Calbindin-positive cells are contained in a caudal domain of nucleus NCM (A); coincidentally, this caudal sub-domain also exhibits high expression levels of the estrogen-synthesizing enzyme aromatase (B, C). From Pinaud et al., 2006.

Confocal images showing song-response (zenk-positive) GABAergic neurons (zGAD65-positive) in nucleus NCM of the adult zebra finch. From Pinaud et al., 2004.